Cold Spring Harbor Protocols

Large-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA.

Round A/Round B Amplification of DNA for Microarrays.

Measuring the Infectious Titer of Recombinant Adenovirus Using Tissue Culture Infection Dose 50% (TCID50) End-Point Dilution and Quantitative Polymerase Chain Reaction (qPCR).

Endoplasmic Reticulum Network Formation with Xenopus Egg Extracts.

Preparation of dsRNAs for RNAi by In Vitro Transcription.

An RNA-Seq Protocol for Differential Expression Analysis.

Detection of an Antigen on an Immunoblot.

Direct Cyanine-dCTP Labeling of DNA for Microarrays.

Ligation and Ligases.

Preparation of siRNA Duplexes.

Calcium Phosphate-Mediated Transfection of Adherent Cells or Cells Growing in Suspension: Variations on the Basic Method.

DNA Transfection by Electroporation.

Blocking of Immunoblots and Incubation with Antibodies: Procedure for Immunoblots Prepared with Whole-Cell Lysates or Purified Proteins.

Cleavage Blastomere Explant Culture in Xenopus.

Cross-Linking Antibodies to Beads with Disuccinimidyl Suberate (DSS).

Blocking Polylysines on Homemade Microarrays.

Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

T7 Linear Amplification of DNA (TLAD) for Microarrays.

Conjugation of Peptides to Thiol-Reactive Gel for Affinity Purification of Antibodies.

RNAi in Mammalian Cells by siRNA Duplex Transfection.

Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.

Microinjection of DNA Constructs into Xenopus Embryos for Gene Misexpression and cis-Regulatory Module Analysis.

Preparing Protein Antigens from Sodium Dodecyl Sulfate-Polyacrylamide Gels for Immunization.

Spun-Column Chromatography.

DNase-seq to Study Chromatin Accessibility in Early Xenopus tropicalis Embryos.

Generating a Three-Dimensional Genome from Xenopus with Hi-C.

Ex Vivo Eye Tissue Culture Methods for Xenopus.

Screening Colonies by Polymerase Chain Reaction (PCR).

Labeling Antibodies Using N-Hydroxysuccinimide (NHS)-Fluorescein.

RNAi in Drosophila S2 Cells by dsRNA Soaking.

Labeling Antibodies Using a Maleimido Dye.

Assessing Antibody Responses to Pathogens or Model Antigens in Xenopus by Enzyme-Linked Immunosorbent Assay (ELISA).

Detecting Horseradish Peroxidase-Labeled Cells.

Detecting Alkaline Phosphatase-Labeled Cells.

Isolation of High-Molecular-Weight DNA from Mouse Tail Tips.

Single-Cell Cloning of Hybridoma Cells by Growth in Soft Agar.

Special Considerations for Making Explants and Transplants with Xenopus tropicalis.

Recovery of DNA from Agarose Gels Using Glass Beads.

Reconstituting Nuclear and Chromosome Dynamics Using Xenopus Extracts.

Measurement of Radioactivity in Nucleic Acids.

Conjugation of Antibodies to Horseradish Peroxidase.

The Use of Cell-Free Xenopus Extracts to Investigate Cytoplasmic Events.

Printing Microarrays.

Observation of Fluorescent Proteins in Living Cells.

Spemann-Mangold Grafts.

Investigating the Cellular and Molecular Mechanisms of Wound Healing in Xenopus Oocytes and Embryos.

Applying Tensile and Compressive Force to Xenopus Animal Cap Tissue.