Current Protocols in Molecular Biology

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

deltaTE: Detection of Translationally Regulated Genes by Integrative Analysis of Ribo‐seq and RNA‐seq Data

Transcriptome‐Wide Mapping of m6A and m6Am at Single‐Nucleotide Resolution Using miCLIP

Pooled CRISPR Screens in Drosophila Cells

Chromatin‐Associated RNA Sequencing (ChAR‐seq)

Single‐Cell RNA‐seq: Introduction to Bioinformatics Analysis

Whole‐Genome Sequencing of Yeast Cells

STARR‐seq and UMI‐STARR‐seq: Assessing Enhancer Activities for Genome‐Wide‐, High‐, and Low‐Complexity Candidate Libraries

Auxin‐Inducible Degron System for Depletion of Proteins in Saccharomyces cerevisiae

CRISPR‐Cas9‐Guided Genome Engineering in Caenorhabditis elegans

Refolding Proteins from Inclusion Bodies using Differential Scanning Fluorimetry Guided (DGR) Protein Refolding and MeltTraceur Web

Growth of E. coli on Solid Media

Two‐Step Co‐Immunoprecipitation (TIP)

Targeted Transcriptional Activation in Plants Using a Potent Dead Cas9–Derived Synthetic Gene Activator

Low‐Input MNase Accessibility of Chromatin (Low‐Input MACC)

Growth of E. coli in Liquid Medium

Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors

RNA Fragmentation and Sequencing (RF‐Seq): Cost‐Effective, Time‐Efficient, and High‐Throughput 3′ mRNA Sequencing Library Construction in a Single Tube

Targeted Profiling of RNA Translation

Probing In Vivo Structure of Individual mRNA 3′ Isoforms Using Dimethyl Sulfate

Digital Droplet PCR for Monitoring Tissue‐Specific Cell Death Using DNA Methylation Patterns of Circulating Cell‐Free DNA

Optimizing Tissue Preservation for High‐Resolution Confocal Imaging of Single‐Molecule RNA‐FISH

Protein Binding to mRNA 3′ Isoforms

In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single‐Cell Resolution